Myelin Basic Protein (MBP, GDB, Golli MBP, Hemopoietic MBP, HMBPR, HUGO, MGC99675, Myelin Deficient, MLD, Myelin A1 Protein, Myelin Membrane Encephalitogenic Protein, Shiverer, SHI, SP)

Cellular Localization: Cytoplasmic side of myelin\n\nApplications: \nSuitable for use in AF, RID, Immunofluorescence, ELISA, Western Blot and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M9758-01G

Size

1ml

Applications

ELISA, IF, IHC, WB

Hosts

Rat

Reactivities

Hum, Mouse, Rat, Bov, GP, Rabbit, Sheep

Form

0.1% Sodium Azide; Tissue Culture Supernatant

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG2a

References

This product has been used in:Groome NP et al. Region-specific immunoassays for human myelin basic protein. J Neuroimmunol 12:253-64 (1986). <PUBMED:2428830> Glynn P et al. Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments. J Neurochem 48:752-9 (1987). <PUBMED:2433395>

Additional Info

The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87. Mapped by Geyson method to DENPW method. Species Crossreactivity: Crossreacts with Human, bovine, Guinea pig, Mouse, Rabbit, Rat and Sheep.