Myeloperoxidase, Human

Myeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59-64kD) and two unglycosylated beta chains (MW 14kD) with total MW 150kD and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers.\n\nSource: \nMyeloperoxidase from leukocytes of purulent human sputum.\n\nMolecular Weight:\n59kD (SDS-PAGE) indicating 2 protein subunits\n129kD (S-200 chromatography)\n\nBiological Activity:\n155U/mg (prior to lyophilization): 1 unit will decompose 1 micromole of H2O2 per minute at pH 7.0 and 25 degrees C using 4-amino-antipyrine as hydrogen donor. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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