Myeloperoxidase (MPO, pANCA)

In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types, at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. It is readily detected in myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythro-leukemia, myeloblastomas, and other hematopoietic disorders. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. \n\nApplications:\nSuitable for use in ELISA and Immunofluorescence. Other applications not tested. \n\nRecommended Dilutions: \nImmunofluorescence: Exhibits a P-ANCA pattern\nOptimal dilution determined by the researcher.\n\nAutoantibody Activity:\nInterpretation of Results: <16EU/ml: Negative\n16-20EU/ml: Equivocal\n>20EU/ml: Positive\n\nAnti-MPO Activity by ELISA: As reported (lot-specific). Current lot value: 96EU/ml\nInterpretation of Results: <4EU/ml: Negative\n4.1-5.9EU/ml: Equivocal\n>6EU/ml: Positive\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M9760

Size

2ml

Applications

ELISA, IF

Reactivities

Hum

Form

Supplied as a powder lyophilized from a solution containing PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.

P Type

Pab

Purity

Fractionated from delipidated, defibrinated and precipitated human serum.

Isotype

IgG

Additional Info

Recognizes human Myeloperoxidase (MPO, pANCA) \nSpecies Crossreactivity: Should detect MPO from most species. Does not recognize PR-3, SS-A, SS-B, Sm, Sm/RNP, SCL and Jo-1 antigens at a 1:1000 dilution in ELISA.