Myosin Light Chain 2, Non-muscle, Recombinant, Human (MLC2, Myosin Regulatory Light Chain, MRLC, RLC, LC20)

Non muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daughter cells. They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages. Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca+2 Calmodulin regulated light chain kinase (MLCK).\n\nActivity: \nAssayed for phosphorylation by MLCK.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Inhibition Assays. Other applications have not been tested.\n\nAmino Acid Sequence:\nMSSKKAKTKT TKKRPQRATS NVFAMFDQSQ IQEFKEAFNM IDQNRDGFID KEDLHDMLAS LGKNPTDAYL DAMMNEAPGP INFTMFLTMF GEKLNGTDPE DVIRNAFACF DEEATGTIQE DYLRELLTTM GDRFTDEEVD ELYREAPIDK KGNFNYIEFT RILKHGAKDK DD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

M9850-09N

Size

2ug

Form

Supplied as a liquid in 20mM HEPES-KOH, pH 7.0, 50mM sodium chloride, 1mM EDTA and 1mM DTT.

Purity

~90% by SDS-PAGE

References

1. Exp. Mol. Med. 38: 180-189 (2006). 2. J. Biol. Chem. 280: 33,083-33,095 (2005). 3. J. Biol. Chem. 280: 23,741-23,747 (2005). 4. Biofactors 21: 387-389 (2004). 5. Exp. Eye Res. 79: 543-551 (2004). 6. Biochem. J. 385: 589-594 (2005).