Myosin Light Chain (MLC, A1 Catalytic, A2 Catalytic, Alkali Myosin Light Chain 1, Alkali Myosin Light Chain 3, C36E6.3, CMH10, DKFZp779C0562, MLC2, MLC-2, MLC-2v, MLC1F, MLC3F, MYL1, MYL2, Myosin Light Chain 1 Alkali Skeletal Fast, Myosin Light Chain 1 Sk

Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions.\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:200. Localizes an epitope on the stress fibers of well spread human and chicken fibroblasts by indirect immunofluorescence.\nWestern Blot: Reacts with the 20kD light chains of SDS, heat-denatured and reduced chicken gizzard, chicken intestine, and porcine stomach myosin.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9850-02C

Size

100ul

Applications

IF, WB

Hosts

Mouse

Reactivities

Hum, Bov, Ch/Bird, Prc, Rabbit

Form

Supplied as liquid, 15mM sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgM

Additional Info

Recognizes chicken Myosin Light Chain at ~20kD. Does not react in Western Blot with intact myosin molecules in their native state. Species Crossreactivity: bovine, human, porcine and rabbit.