Myosin VIIa (Myosin 7a, Myo7a, MYOVIIA, Deafness Autosomal Dominant 11, DFNA11, Deafness Autosomal Recessive 2, DFNB2, MYU7A, NSRD2, Usher Syndrome 1B, Ush1B)

Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMouse testes tissue extract\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9848-03H

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Prc

Form

Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Liu X et al. Myosin VIIa, the product of the Usher 1B syndrome gene, is concentrated in the connecting cilia of photoreceptor cells. Cell Motil Cytoskeleton 37:240-52 (1997). 2. Hasson T et al. Expression in cochlea and retina of myosin VIIa, the gene product defective in Usher syndrome type 1B. Proc Natl Acad Sci U S A 92:9815-9 (1995).

Additional Info

Recognizes Myosin VIIa from mouse tissues as well as the recombinant protein. Detects recombinant mouse myosin VIIa overexpessed in Sf9 insect cell lysate.