Myostatin Fusion Protein, Recombinant, Human, Propeptide (Growth Differentiation Factor 8, MSTN, gdf8)

Myostatin (GDF-8), a member of the TGFbeta superfamily, is a potent and specific negative regulator of skeletal muscle mass. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.\n\nDescription: 28kD protein containing 243 amino acid residues of the human Myostatin Propeptide and 5 additional amino acid residues. MG NENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN\n\nSpecificity: \nThe amino acid sequence of the recombinant human Myostatin Propeptide is 100% homologous to the amino acid sequence of the human Myostatin Propeptide without signal sequence.\n\nApplications: \nWestern blotting, ELISA\n\nSolubility: \nAdd 0.2 ml of d H2O and let the lyophilized pellet dissolve completely.\n\nStability: \nStore lyophilized protein at -20 degrees C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degrees C for a limited period of time. The lyophilized protein remains stable until the expiry date when stored at -20 degrees C.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

M9875-01C

Size

5ug

Applications

ELISA, WB

Form

Supplied as a lyophilized powder in 20mM Tris, 20mM sodium chloride, pH 8.0.

Purity

(same/more than) 95% (SDS-PAGE); Two-step procedure using size exclusion chromatography before and after refolding.

References

1. Work-induced changes in skeletal muscle IGF-1 and myostatin gene expression in uremia.\n\nKidney Int 2006 Jun 28;\n\n2. Myostatin regulation of muscle development: Molecular basis, natural mutations, physiopathological aspects.\n\nExp Ce ll Res 2006 May 3;\n\n3. Increased myostatin synthesis in rat gastrocnemius muscles under high-protein diet.\n\nInt J Sport Nutr Exerc Metab 2006 Apr;16(2):153-65\n\n4. Production of a monoclonal anti-myostatin antibody and the effects of in ovo administration of the antibody on posthatch broiler growth and muscle mass.\n\nPoult Sci 2006 Jun;85(6):1062-71\n\n5. Myostatin Regulates Cardiomyocyte Growth Through Modulation of Akt Signaling.\n\nCirc Res 2006 Jun 8;\n\n6. A mutation creating a potential illegitimate microRNA target site in the myostatin gene affects muscularity in sheep.\n\nNat Genet 2006 Jul;38(7):813-818