Myostatin Propeptide

Myostatin (GDF 8) is expressed uniquely in human skeletal muscle as a 12kD mature glycoprotein consisting of 109 aa residues and secreted into plasma. Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70%) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: Indirect\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1906: IgY, Chicken Pab Rb x Ch \nI1906-10: IgY, Chicken (HRP) Rb x Ch \nI1906-12: IgY, Chicken (FITC) Rb x Ch \nI1906-14: IgY, Chicken (AP) Rb x Ch \n\nAmino Acid Sequence:\nMGNENSEQKE NVEKEGLCNA CTWRQNTKSS RIEAIKIQIL SKLRLETAPN ISKDVIRQLL PKAPPLRELI DQYDVQRDDS SDGSLEDDDY HATTETIITM PTESDFLMQV DGKPKCCFFK FSSKIQYNKV VKAQLWIYLR PVETPTTVFV QILRLIKPMK DGTRYTGIRS LKLDMNPGTG IWQSIDVKTV LQNWLKQPES NLGIEIKALD ENGHDLAVTF PGPGEDGLNP FLEVKVTDTP KRSRRKLN\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M9875-03

Size

50ug

Applications

ELISA, WB

Hosts

Ch/Bird

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 50ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

1. Wolfman, N.M., et al., Proc. Natl. Acad. Sci. USA 100(26): 15,842-15,846 (2003). 2. Hill, J.J., et al., Mol. Endocrinol. 17(6): 1144-1154 (2003). 3. Hill, J.J., et al., J. Biol. Chem. 277(43): 40,735-40,741 (2002). 4. Lee, S.J. & McPherron, A.C., Proc. Natl. Acad. Sci. USA 98(16): 9306-9311 (2001).

Additional Info

Recognizes human Myostatin Propeptide.