MYT1, phosphorylated (Thr495) (Membrane-associated Tyrosine-and Threonine-specific cdc2-inhibitory Kinase, Myt1 Kinase, PKMYT1) (Biotin)

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Dot Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:50-1:100\nDot Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M9800-80-Biotin

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004). 2.Nakajima, H., et al., J. Biol. Chem. 278(28):25277-25280 (2003). 3.Passer, B.J., et al., Proc. Natl. Acad. Sci. USA 100(5):2284-2289 (2003). 4.Okumura, E., et al., Nat. Cell Biol. 4(2):111-116 (2002). 5.Booher, R.N., et al., J. Biol. Chem. 272(35):22300-22306 (1997).

Additional Info

Recognizes human MYT1 when phosphorylated at Thr495.