N-Acetylneuraminic Acid (NANA, Sialic Acid) BioAssay(TM) Kit

Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid. Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been show recently that sialic acid level may associate with developmental and pathological stages. \n\nSialic Acid BioAssay(TM) Kit provides a simple and convenient means of measuring free Sialic Acid in a variety of biological samples. The kit utilizes an enzyme coupled reaction in which free sialic acid is oxidized resulting in development of the Oxi-Red probe to give fluorescence (Ex/EmS5/587nm) and absorbance (O.D.W0nm). The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1uM concentration.\n\nKit Components:\n1. Sialic Acid Assay Buffer: 1x25ml \n2. Sialic Acid Probe: lyophilized \n3. DMSO, Anhydrous: 1x400ul \n4. Sialic Acid Converting Enzyme: lyophilized \n5. Sialic Acid Development Mix: lyophilized \n6. Sialic Acid Standard (10umol): lyophilized \n\nStorage and Stability:\nStore kit at -20 degrees C. Protect from light. Allow Assay Buffer to warm to room temperature \nbefore use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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