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NADP/NADPH Quantification BioAssay(TM) Kit

Cat no: N0009-55

NADP/NADPH Quantification BioAssay(TM) Kit

Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD+/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450nm.\n\nKit Components:\nNADP/NADPH Extraction Buffer 1x50ml\nNADP Cycling Buffer 1x15ml\nNADP Cycling Enzyme Mix 1x1vial\nNADPH Developer 1x1vial\nStop Solution 1x1.2ml\nNADPH Standard (MW:833.36) 1x166.7ug.\n\nStorage and Stability:\nStore the kit at -70 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N0009-55

Size

100Tests

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