NAGA (Alpha-N-acetylgalactosaminidase, Alpha-galactosidase B)

NAGA is a lysosomal a-N-acetylgalactosaminidase that cleaves non-reducing a-N-acetylgalactosaminyl moieties from glycoconjugates. Mature NAGA has 394aa and is trafficked to the lysosome via the mannose-6-phosphate receptor-mediated pathway. The enzyme is a retaining exoglycosidase, where both the substrate and product of the enzymatic reaction have the same anomeric configuration. Deficiency in NAGA results in increased urinary excretion and tissue accumulation of glycopeptides and oligosaccharides containing terminal aNacetylgalactosaminyl moieties, manifesting as Schindler's disease, an autosomal recessive disease with neuroaxonal dystrophy and other neurological symptoms. The enzyme can be used to remove a-N-acetylgalactosaminyl residues present on red blood cells thus converting blood type A to blood type O.\n\nApplications:\nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1ug/ml\nImmunocytochemistry: 5-15ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0528-11

Size

100ug

Applications

ICC, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wang, A.M. et al. (1990) J. Biol. Chem. 265:21859. 2. Sweeley, C.C. et al. (1983) Arch. Biochem. Biophys. 223:158. 3. Garman, S.C. et al. (2002) Structure. 10:425. 4. Eng, C.M. et al. (2001) N. Engl. J. Med. 345:9. 5. Wang, A.M. et al. (1990) J. Clin. Invest. 86:1752. 6. Liu, Q.P. et al. (2007) Nature Biotechnol. 25:454. 7. Calcutt, M. J. et al. (2002) FEMS Microbiol. Lett. 214:77. 8. Zhu, A. et al. (1996) Arch. Biochem. Biophys. 327:324.

Additional Info

Recognizes human NAGA. Species Crossreactivity: Recombinant human aGalactosidase\nA (>2%).