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Nanog, phosphorylated (Ser285) (Homeobox Transcription Factor Nanog, Homeobox Protein NANOG, hNanog) (Biotin)

Cat no: N0019-01N-Biotin

Nanog, phosphorylated (Ser285) (Homeobox Transcription Factor Nanog, Homeobox Protein NANOG, hNanog) (Biotin)

NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.\n\nApplications: \nSuitable for use in ELISA and Dot Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nDot Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N0019-01N-Biotin

Size

200ul

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Kochupurakkal,B.S., Biochem. Biophys. Res. Commun. 365(4), 846-850 (2008). 2. Freberg,C.T., Mol. Biol. Cell 18(5), 1543-1553 (2007).

Additional Info

Recognizes human NANOG when phosphorylated at Ser285.

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