Nerve Growth Factor, 7S (NGF)

Dose for significant outgrowth of neurite using PC-12 rat pheochromocytoma cells: 10ng/ml. 7S NGF has a variety of effects on sensory and sympathetic neuron growth and development.3 7S NGF is also needed for sympathetic nerve cells development and maintenance.\n\nBiological Activity: \nDose for significant outgrowth of neurite using PC-12 rat pheochromocytoma cells: 10ng/ml. 7S NGF has a variety of effects on sensory and sympathetic neuron growth and development. 7S NGF is also needed for sympathetic nerve cells development and maintenance.\n\nExtinction Coefficient\n( E1%, 280nm, pH 7.0): 14.2 \n\nSterility: \nSterilized through a 0.2um membrane filter and packaged aseptically.\n \nNGF Assay: \n1. Coat 6 well dishes with rat tail collagen type I, at a concentration of 2.5-5.0ug/cm2. Incubate for one hour at 37 degrees C. Aspirate and rinse 2 times with PBS before adding cell suspension. \n2. Prepare a cell suspension to yield 2x10e4 cells/ml in RPMI-1640/10% horse serum/ 5% FBS. Add 2mls of cell suspension to each well. \n3. Prepare NGF as described above under Rehydration to a final concentration of 1ug/ml. Add appropriate amounts of NGF to test at 0, 5, 10 and 20ng/ml final concentration. \n4. Incubate plates at 37 degrees C for 3-5 days.\n5. Record neurite outgrowth at each NGF concentration. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2050-06

Size

25ug

Form

Supplied as a liquid.

Purity

Purified by gel filtration. Does not contain detectable amounts of EGF

References

1. Bocchini, V. and P.U. Angeletti, Proc. Natl. Acad. Sci. 64: 787-794, 1969. 2. Bocchini, V., Eur. J. Biochem. 15: 127-131, 1970. 3. Levi-Montalcini, R. and P.U. Angeletti, Physiol. Rev. 48: 534-569, 1968. 4. Marx, J.L., Science 232: 1341-1342, 1986.