Neuro?lament L,H, 70kD, 200kD (NF-L+H)

Neurofilaments (NFs), the intermediate filaments (IFs) specific to neurons, are the predominant cytoskeletal elements in many nerve cells. They are believed not only to confer mechanical stability, but also to play a role in determining axonal caliber. Human NFs are composed of three protein subunits, NF-L,NF-M and NF-H, which have mol. wts predicted from their gene sequences of-62,000,-102,000 and-110,000 respectively. Each type of NFs contains a central a-helical 'rod' domain of-310aa, characterized by a heptad repeat in the aa sequence in which the first and fourth residues are apolar. Perikaryal NFs are predominantly unphosphorylated, while axonal NFs are highly phosphorylated. Defects in NFL are the cause of Charcot-Marie-Tooth disease type 1F while defects in NFH are a cause of susceptibility to amyotrophic lateral sclerosis. The gene bank accession no of NF-L, NF-M and NF-H are NP_006149.1, NP_066554.2 and NP_005373.1 respectively.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:50-1:100\nImmunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nBrain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N2160-08B

Size

500ul

Applications

ICC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Osborn et al. Virchows arch 40: 141, 1984. 2. Osborn et al. Am J Pathol 122: 433, 1986. 3. Van Muijen et al. Human Pathol 16: 590, 1985. 4. Julien et al. Biochim Biophys Acta 909: 10, 1987.

Additional Info

Recognizes the 70 and 200kD component of human Neurofilaments. It stains neurons, neuronal processes and peripheral nerves. This antibody reacts with some neuroblastomas and gangliomas but not with other non-neuronal tumors.