Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, CMT1F, CMT2E, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68kD (NFL), 125kD (NFM) and 200kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament rotein, Light Chain (NFL). It is one of the most abundant cytoskeletal omponents of the neuron.Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry: 2-4ug/ml detects NF68 in formalin fixed paraffin embedded tissues and formalin or acetone fixed frozen tissues.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1.2% sodium acetate or sterile PBS.. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2160-01H2

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Prc

Form

Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

1. Zhu, Q., et al. Exp. Neurol. 148: 299-316, 1997. 2. Jordanova, A., et al. Brain 126: 590-597, 2003. 3. Previtali, S. C., et al. Hum. Molec. Genet. 12: 1713-1723, 2003.

Additional Info

Recognizes porcine Neurofilament L, 68kD. Species Crossreactivity: human, rat.