Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at ~68kD. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.\n\nApplications:\nSuitable for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:100-1:500\nImmunohistochemistry: Frozen and formalin fixed sections\nWestern Blot: 1:5000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031403

Size

500ul

Applications

ICC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Prc

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG1,k

References

1. Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46 (2000). 2. Edson KJ, Linck RW, Letourneau PC. Identification of a tektin-like protein associated with neurofilaments in the developing chick nervous system. J Neurosci Res. 30:105-15 (1991). 3. Bongarzone ER, Foster L, Byravan S, Casaccia-Bonnefil P, Schonmann V, Campagnoni AT. Two neuronal cell lines expressing the myelin basic protein gene display differences in their in vitro survival and in their response to glia. J Neurosci Res. 54:309-19 (1998). 4. Evans J. et al. Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 (2002). 5. Shaw G, Morse S, Ararat M, Graham FL. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 16:869-71 (2002). 6. Zhang L, Gavin T, DeCaprio AP, LoPachin RM. Gamma-diketone axonopathy: analyses of cytoskeletal motors and highways in CNS myelinated axons. Toxicol Sci. 117:180-9 (2010). 7. Paulussen M, Jacobs S, Van der Gucht E, Hof PR, Arckens L. Cytoarchitecture of the mouse neocortex revealed by the low-molecular-weight neurofilament protein subunit. Brain Struct Funct. 216:183-99 (2011).

Additional Info

Recognizes porcine NF-L. Species Crossreactivity: human, bovine, mouse, rat, avian and all other mammals.