NF-kB (human p50/p65) Combo Transcription Factor Assay Kit

The NF-kB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-kB. Acting as dimers, these transcription factors bind to kB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-kB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman's NF-kB (human p50/p65) Combo Transcription Factor Assay is a non-radioactive, sensitive method for detecting p50 and p65 transcription factor DNA binding activity in nuclear extracts.

Prices direct from Cayman Chemical Company

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

10011223

Form

96 Well

P Type

Assay Kits|NF-.kappa.B|Intracellular Signaling

Weight

0

Storage Temp

-80

Shipping Temp

-80

Additional Info

The NF-.kappa.B/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-.kappa.B. Acting as dimers, these transcription factors bind to .kappa.B sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-.kappa.B dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman's NF-.kappa.B (human p50/p65) Combo Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts. A specific double stranded DNA (dsDNA) sequence containing the NF-.kappa.B response element is immobilized onto the wells of a 96-well plate. NF-.kappa.B contained in a nuclear extract, binds specifically to the NF-.kappa.B response element. NF-.kappa.B p50 and p65 are detected by addition of specific primary antibodies directed against either NF-.kappa.B p50 or p65. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.