NF-kB (human p50) Transcription Factor Assay Kit

The NF-kB/Rel family of transcription factors is comprised of several structurally-related proteins that form homodimers and heterodimers and include p50/p105, p52/p100, RelA (p65), and c-Rel/NF-kB. Acting as dimers, these transcription factors bind to kB sites on DNA, thereby regulating expression of target genes. The most common Rel/NF-kB dimer in mammals contains p50-RelA (p50/p65) heterodimers and is an attractive target for potential therapeutics in human inflammation and certain other diseases. Cayman's NF-kB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting human NF-kB (p50) DNA binding activity in nuclear extracts and whole cell lysates. It will not cross-react with NF-kB (p65).

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SPECIFICATIONS

Catalog Number

10006912

Form

96 Well

P Type

Assay Kits|NF-.kappa.B|Intracellular Signaling

Weight

0

Storage Temp

-80

Shipping Temp

-80

Additional Info

Cayman's NF-kB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kB response element is immobilized to the wells of a 96-well plate. NF-kB contained in a nuclear extract, binds specifically to the NF-kB response element. NF-kB (p50) is detected by addition of specific primary antibody directed against NF-kB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The Cayman Chemical NF-kB (human p50) Transcription Factor Assay detects human NF-kB (p50). It will not cross-react with NF-kB (p65).