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Products NF-kB (p65) Transcription Factor Assay Kit
| Catalogue number: | 10007889 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|NF-.kappa.B|Intracellular Signaling |
| Shipping temp: | -80 |
| Storage temp: | -80 |
| Additional info: | Cayman's NF-.kappa.B (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-.kappa.B response element is immobilized to the wells of a 96 well plate. NF-.kappa.B contained in a nuclear extract, binds specifically to the NF-.kappa.B response element. NF-.kappa.B (p65) is detected by addition of specific primary antibody directed against NF-.kappa.B (p65). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. Cayman's NF-.kappa.B (p65) Transcription Factor Assay detects human NF-.kappa.B (p65). It will not cross-react with NF-.kappa.B (p50). |
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