NFkB Activating Kinase, NT (Nuclear Factor kappa B, TANK-Binding Kinase 1, NAK, TBK1)

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor?associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumor promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, and Flow Cytometry. Other applications not tested.Product Type: Mab\nIsotype: IgG\nClone No: 6k282\nHost: rabbit\nSource: Human\nForm: Supplied as a liquid.\nPurity: Supernatant\nImmunogen: A synthetic peptide corresponding to residues in the N-term of human NAK \nSpecificity: Recognizes human NAK \n\nRecommended Dilution:\nWestern Blot: 1:1,000\nImmunohistochemistry: 1:50\nImmunocytochemistry: 1:250-500\nFlow Cytometry: 1:40\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts