NFkB, c-Rel (NF-kB, Nuclear Factor kappa B)

Transcription factors of the nuclear factor k B (NF-kB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-kB1 (p105/p50) and NF-kB2 (p100/p52). Both p105 and p100 are proteolyti- \ncally processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-kB is sequestered in the cytoplasm by IkB inhibitory proteins (3-5). NF-kB-activating agents can induce the phosphorylation of IkB proteins, targeting them for rapid degradation through an ubiquitin-proteasome pathway and releasing NF-kB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKalpha (IKK1) regulate the phosphorylation and processing of NF-kB2 (p100) to produce p52, which is then translocated to the nucleus (9-11). \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, \n 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2303-10D

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 0.1%, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79, 2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20, 3. Haskill, S. et al. (1991) Cell 65, 1281-9, 4. Thompson, J.E. et al. (1995) Cell 80, 573-82, 5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26, 6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83, 7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63, 8. Chen, Z.J. et al. (1996) Cell 84, 853-62, 9. Senftleben, U. et al. (2001) Science 293, 1495-9, 10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85, 11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

Additional Info

Recongnizes endogenous levels of human total c-Rel protein. Species crossreactivity: mouse, rat. Sequence Homology: monkey, canine.