ST6GALNAC5 (Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5, GD1 alpha Synthase, GalNAc alpha-2,6-sialyltransferase V, ST6GalNAc V, ST6GalNAcV, Sialyltransferase 7E, SIAT7-E, SIAT7E)

Gangliosides are acidic glycosphingolipids that contain one or more sialic acid residues. They are abundant in the nervous system, where they play crucial modulatory roles in cellular recognition, interaction, adhesion, and signal transduction, particularly during early developmental stages. The expression of gangliosides in the nervous system is developmentally regulated through various sialyltransferases. ST6GALNAC5 is a sialyltransferase involved in the biosynthesis of ganglioside GD1a (NeuAca2,3GalB1,3GalNAcB1,4(NeuAca2, 3)GalB1,4GlcB1Cer) from GM1b (NeuAca2,3GalB1,3GalNAcB1,4GalB1,4GlcB1Cer), and its expression is restricted to the brain. ST6GALNAC5 has been identified as a key player in the metastasis of breast cancer cells to the brain by potentially enabling the cancer cells to cross the blood-brain barrier. The recombinant ST6GALNAC5 was active on fetuin from fetal calf serum when assayed using a phosphatase-coupled method suggesting the substrate specificity of ST6GALNAC5 may require further characterization.\n\nApplications:\nSuitable for use in Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 8-25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0550-08

Size

100ug

Applications

ICC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2A

References

1. Kolter, T. et al. (2002) J. Biol. Chem. 277:25859. 2. Yu, R.K. et al. (2008) Glycoscience DOI: 10.1007/9783540304296_41. 3. Okajima, T. et al. (1999) J. Biol. Chem 274:30557. 4. HarduinLepers, A. et al. (2005) Glycobiology 15:805. 5. Bos, P.D. et al. (2009) Nature 459:1005. 6. Arshad, F. et al. (2011). Patholog. Res. Int. DOI: 10.4061/2011/920509. 7. Wu, Z.L. et al. (2010) Glycobiology DOI: 10.1093/glycob/cwq187.

Additional Info

Recognizes human ST6GALNAC5. No crossreactivity with recombinant human ST6GALNAC4 or rhST6GALNAC6.