NFkB, p65 (NF-kB, Nuclear Factor kappa B, MGC131774, NFKB3, Nuclear Factor NF-Kappa-B p65, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells, RelA, Transcription Factor p65, v-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A)

NFkB was originally identified as a factor that binds to the immunoglobulin K-light chain enhancer in B cells. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkBa.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:5000\nELISA: 1:50,000-1:100,000\nWestern Blot: 1:1000-1:5000\nImmunohistochemistry: 1:200-1:600\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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