Nitrate/Nitrite Fluorometric Assay Kit

Cayman's Nitrate/Nitrite Fluorometric Assay provides a convenient method for the quantitation of low levels of nitrate and nitrite in biological samples (particularly tissue culture medium). Previously undetectable concentrations of nitrate/nitrite in a variety of samples can be accurately quantitated using this kit as it extends the lower limit of detection by 20-fold over our colorimetric version (Item No. 780001). The minimum detectable quantity of NO2/NO3 is ~50 nM.

Prices direct from Cayman Chemical Company

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

780051

Form

192 Well

P Type

Small Molecule Detection (Non-EIA)|Assay Kits

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Nitric Oxide (NO) is produced in trace quantities by neurons, endothelial cells, platelets, and neutrophils in response to homeostatic stimuli. This NO is scavenged rapidly (t1/2 = 4 s) and acts in a paracrine fashion to transduce cellular signals. NO interacts with the HEME prosthetic group of guanylate cyclase, activating the enzyme and leading to increased cGMP levels. Other cells (macrophages, fibroblasts, and hepatocytes) also produce NO in micromolar concentrations in response to inflammatory or mitogenic stimuli. These very high levels of NO lead to the formation of peroxynitrite, destruction of iron-sulphur clusters, thiol nitrosation, and nitration of protein tyrosine residues. Thus, the amount of NO produced in different biological systems can vary over several orders of magnitude and its subsequent chemical reactivity is diverse. The final products of NO in vivo are nitrite (NO2-) and nitrate (NO3-). Relative proportions of NO2- and NO3- within a sample are variable and cannot be predicted with certainty. Therefore, the best index of total NO production is the sum of both of these products. Cayman Chemical's Nitrate/Nitrite Fluorometric Assay Kit provides a convenient method for the quantitation of low levels of NO2- and NO3- in biological samples (particularly tissue culture medium). Previously undetectable concentrations of nitrate/nitrite in a variety of samples can be accurately quantitated using this kit as it extends the lower limit of detection by 20-fold over our popular colorimetric version. The minimum detectable quantity of NO2/NO3 is ~50 nM.