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Products Nitric Oxide Cell-Based Assay Kit
| Catalogue number: | 10009419 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits |
| Shipping temp: | -20 |
| Storage temp: | -20 |
| Additional info: | Nitric oxide (NO) is a multifunctional cell signaling molecule produced through the action of nitric oxide synthase (NOS). Cayman's Nitric Oxide Fluorometric Cell-Based Assay Kit detects NO in living cells by using fluorescein amine methyl ester (FA-OMe). When the non-fluorescent FA-OMe senses free NO in living cells it forms an intensely fluorescent deamination product, dA-FA-OMe whose fluorescence is directly proportional to the NO being produced by cells. dA-FA-OMe fluorescence can also be used to indirectly examine the NOS activity responsible for NO production. The NOS inhibitor, L-NAME, is also included for this purpose. This assay can be used to directly examine the effect of drugs, cytokines, or other experimental agents on NO production in cultured cells. |
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