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Products Nitrotyrosine Monoclonal Antibody - Biotinylated
| Catalogue number: | 10006966 |
| Hosts: | Mouse |
| Applications: | Immunohistochemistry, Immunoprecipitation, Western Blot |
| Weight: | 0 |
| Form: | 100 microg |
| Antigen: | peroxynitrite-treated KLH |
| P type: | Antibodies |
| Shipping temp: | -20 |
| Storage temp: | -20 |
| Additional info: | Nitric oxide (NO) is a product of the enzymatic conversion of arginine to citrulline by NO synthase. NO reacts rapidly with superoxide (6.7 x 109 M-1sec-1) to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo. Cayman Chemical's biotinylated nitrotyrosine monoclonal antibody can be used for the species-independent detection of nitrotyrosine using a variety of immunochemical techniques. Biotinylation of the antibody allows for detection using avidin-enzyme or avidin-fluorophore conjugates and provides approximately 2-fold better sensitivity compared to the unbiotinylated antibody (Catalog No. 189542). |
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