Nourseothricin (NTC, Nurseotricin, Nourseotricin, clonNAT)

A group of antibiotic substances produced by Streptomyces variants. They are composed of an aminosugar and different polypeptide chains and may have broad spectrum antimicrobial and some antiviral properties. Streptothricin antibiotic Nourseothricin (NTC) is used for a broad spectrum of viruses, bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, plants and many more. Selection of recombinant strains is based on inactivation of NTC by monoacetylation of b-amino group of the b-lysine by Nouresothricin N-acetyltransferase the product of the sat1 gene.\n\nApplications:\nSuitable for use in selection of recombinant Leishmania strains. Other applications not tested.\n\nRecommended Dilutions: \nAdded to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher.\n\nSelection:\nFor selection of recombinant Leishmania strains Nourseothricin NTC is added to the growth medium to a final concentration of 100ug/ml.\n\nReconstitution: \nReconstitute with ddH2O to 100mg/ml, sterile filter and desiccant. Maximal solubility is 1g/ml.\n\nTable 1:\n* MIC: Minimal inhibitory concentration\n** IC50: Concentration inhibiting growth by 50%\n\nGroup Species MIC* Selection conc. \n ug/ml ug/ml\nGram-negative bacteria \nEscherichia coli 2-12 50\nFrancisella tularensis - 5\nPseudomonas aeruginosa 50 100\nGram-positive bacteria \nBacillus subtilis 5 50\nEnterococcus faecium 8-256 500\nStaphylococcus aureus -12 50\nStreptomycetes \nStreptomyces lividans 6 100\nYeast \nCandida albicans 200 250-450\nCryptococcus neoformans - 100\nHansenula polymorpha - 100\nPichia pastoris - 100\nSaccharomyces cerevisiae 25 100\nFilamentous fungi \nAcremonium chrysogenum - 25\nSordaria macrospora - 50\nUstilago maydis - 75\nProtozoa Leishmania tarentolae - 100\nPhytomonas serpens - 100\nPlasmodium falciparum 75* -\nToxoplasma gondii - 500\nPlants \nArabidopsis thaliana 20 200\nOryza sativa 20 200\n\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5375-74

Size

25mg

Form

Supplied as a non-sterile powder. Reconstitute with ddH2O to 100mg/ml, sterile filter and desiccant. Maximal solubility is 1g/ml.

References

1. Cox, et al., Infect. Immunity 71: 173 (2003). 2. Gold, et al., Gene 142: 225 (1994). 3. Goldstein, et al., Yeast 15: 1541 (1999). 4. Hamano, et al., J. Biol. Chem. 281: 16,842 (2006). 5. Hentges, et al., Yeast 22: 1013 (2005). 6. Jacobs, et al., Nature Protocols 4: 58 (2009). 7. Ji, et al., J. Antibiot. 60: 739 (2007). 8. Joshi, et al., Gene 156: 145 (1995). 9. Kloti, et al., Selectable marker in plants. United States Patent 6696621 (2004). 10. Kojic, et al., Can. J. Microbiology 46: 333 (2000). 11. K