Nourseothricin (NTC, Nurseotricin, Nourseotricin, clonNAT)

A group of antibiotic substances produced by Streptomyces variants. They are composed of an aminosugar and different polypeptide chains and may have broad spectrum antimicrobial and some antiviral properties. Streptothricin antibiotic Nourseothricin (NTC) is used for a broad spectrum of viruses, bacteria and other unicellular or complex organisms. Preferred selection antibiotic for genetically modified Gram-positive and Gram-negative bacteria, yeast, filamentous fungi, protozoa, plants and many more. Selection of recombinant strains is based on inactivation of NTC by monoacetylation of b-amino group of the b-lysine by Nouresothricin N-acetyltransferase the product of the sat1 gene. Applications: Suitable for use in selection of recombinant Leishmania strains. Other applications not tested. Recommended Dilutions: Added to growth medium to a final concentration of 100ug/ml. Optimal dilutions to be determined by the researcher. Selection: For selection of recombinant Leishmania strains Nourseothricin NTC is added to the growth medium to a final concentration of 100ug/ml. Reconstitution: Reconstitute with ddH2O to 100mg/ml, sterile filter and desiccant. Maximal solubility is 1g/ml. Table 1: * MIC: Minimal inhibitory concentration ** IC50: Concentration inhibiting growth by 50% Group Species MIC* Selection conc. ug/ml ug/ml Gram-negative bacteria Escherichia coli 2-12 50 Francisella tularensis - 5 Pseudomonas aeruginosa 50 100 Gram-positive bacteria Bacillus subtilis 5 50 Enterococcus faecium 8-256 500 Staphylococcus aureus -12 50 Streptomycetes Streptomyces lividans 6 100 Yeast Candida albicans 200 250-450 Cryptococcus neoformans - 100 Hansenula polymorpha - 100 Pichia pastoris - 100 Saccharomyces cerevisiae 25 100 Filamentous fungi Acremonium chrysogenum - 25 Sordaria macrospora - 50 Ustilago maydis - 75 Protozoa Leishmania tarentolae - 100 Phytomonas serpens - 100 Plasmodium falciparum 75* - Toxoplasma gondii - 500 Plants Arabidopsis thaliana 20 200 Oryza sativa 20 200 Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.