Nrf2 (N-Term) Polyclonal Antibody

Antigen: human Nrf2 N-terminal amino acids 18-29 . Host: rabbit . Cross Reactivity: (+) human, mouse, and rat Nrf2 . Application(s): FC, ICC, and WB . This N-terminal Nrf2 polyclonal antibody detects both poly-ubiquitinated Nrf2 at 90 kDa and native Nrf2 at 67 kDa. Nrf2 forms a heterodimer with a small Maf protein and binds to the antioxidant response element in the upstream promoter region of many antioxidative genes. Under normal, unstressed conditions, Nrf2 is sequestered in the cytoplasm where it is bound by Keap1 and cullin 3. Certain stressors disrupt Keap 1 binding and prevent ubiquitination, leading to subsequent translocation into the nucleus.

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SPECIFICATIONS

Catalog Number

14114

Applications

FC, ICC, WB

Hosts

Rabbit

Form

1 ea

P Type

Antibodies|Intracellular Signaling

Weight

0

Antigen

human Nrf2 N-terminal amino acids 18-29

Storage Temp

-20

Shipping Temp

-20

Additional Info

This N-terminal Nrf2 polyclonal antibody detects both poly-ubiquitinated Nrf2 at 90 kDa and native Nrf2 at 67 kDa. Nrf2 is a transcription factor that forms a heterodimer with a small Maf protein and binds to the antioxidant response element in the upstream promoter region of many antioxidative genes. Under normal, unstressed conditions, Nrf2 is sequestered in the cytoplasm where it is bound to and ubiquitinated by Kelch like-ECH-associated protein 1 (Keap1) and cullin 3. Certain inflammatory, environmental, or oxidative stressors disrupt Keap 1 binding and prevent ubiquitination. This leads to a buildup of Nrf2 in the cytoplasm, and subsequent translocation into the nucleus.