NRG1, Recombinant, Human (GGF, HGL, HRGA, NDF, SMDF, Pro-neuregulin-1, Membrane-bound Isoform, Neuregulin-1, Acetylcholine Receptor-inducing Activity, Breast Cancer Cell Differentiation Factor P45, Glial Growth Factor, Heregulin, Neu Differentiation Facto

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-beta1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-beta1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-beta1 (HRG1-beta1) is a 7.5kD polypeptide consisting of only the EGF domain of heregulin-1, 65aa.\n\nSource:\nRecombinant protein corresponding to human Heregulin-B1, expressed in E. coli.\n\nBiological Activity:\nDetermined by the dose-dependent stimulation of the proliferation of human MCF-7 cells. The ED50 was found to be 0.5ng/ml. \n\nSpecific Activity:\n2x10e6units/mg.\n\nEndotoxin:\n<0.1ng/ug (1EU/ug)\n\nAA Sequence:\nSHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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