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NSE, Recombinant, Human (Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Enolase 2, Neural Enolase, Neuron-specific Enolase, ENO2)

Cat no: N2175-01R

NSE, Recombinant, Human (Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Enolase 2, Neural Enolase, Neuron-specific Enolase, ENO2)

NSE is the gamma/gamma isoform of the glycolytic enzyme enolase and is expressed primarily in neurons, in normal and neoplastic neuroendocrine cells . NSE is a highly soluble cytoplasmic protein that is readily secreted into the CSF and serum following tissue damage. NSE shows neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons and binds in a calcium-dependent manner to cultured neocortical neurons promoting cell survival.\n\nSource: \nRecombinant corresponding to aa2-434 from mature human NSE fused to 6x His-tag at N-terminal, expressed in E. coli.\n\nApplications:\nSuitable for use in Western Blot as a control. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N2175-01R

Size

10ug

Applications

WB

Form

Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Purity

Purified (SDS-PAGE)

References

1. Kaiser, E. et al.,(1989) Clin. Chim. Acta. 183 (1):13-31.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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