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Nsr1p

Cat no: 031415

Nsr1p

Nsr1p is localized in the nucleolus and binds to telomeric single-stranded oligonucleotide repeat sequence of the type and binds to the single-stranded telomeric repeat sequence (TG1-3)n. It has a role in ribosomal RNA maturation and possibly in transport of proteins into the nucleus.\n\nApplications:\nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: For yeast cells, try diluted 1:500-1:5000.\nWestern Blot: For yeast protein samples (cell and nuclear lysates), try diluted 1:10,000, followed by chemiluminescent detection (ECL). For non-ECL Western detection methods, try diluted 1:1000-1:5000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

031415

Size

500ul

Applications

IF, WB

Hosts

Mouse

Form

Supplied as a liquid in 10mM sodium azide.

P Type

Mab

Purity

Sterile-filtered cell culture fluid from an Integra CL-350 biochamber

Isotype

IgG1

References

1. Lin JJ and Zakian VA. Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro. Nucleic Acids Res 22:4906-13 (1994).

Additional Info

Recognizes yeast Nsr1p.

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