NT-3, Recombinant, Human (Neurotrophin 3, Neurotrophin-3, NT3, NTF3, HDNF, MGC129711, Nerve Growth Factor 2, NGF2, NGF-2, Neurotrophic Factor)

Source:\nRecombinant corresponding to 119aa of human Neurotrophin-3 expressed in E.coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~13.6kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent induction of choline acetyl transferase in rat basal forebrain primary septal culture was found between 20-50nug/ml\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N6000-06A

Size

2ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. [Subcloning of human neurotrophin-3 gene and construction of its genetically engineered cell model]. Di Yi Jun Yi Da Xue Xue Bao 2004 Jul;24(7):805-8. 2. [Expression and biological activity of human neurotrophin-3 in olfactory ensheathing cells mediated by retroviral vector]. Shi Yan Sheng Wu Xue Bao 2003 Feb;36(1):5-12. 3. Tolerability of recombinant-methionyl human neurotrophin-3 (r-metHuNT3) in healthy subjects. Muscle Nerve 2000 Feb;23(2):189-92. 4. Renaturation of recombinant human neurotrophin-3 from inclusion bodies using a suppressor agent of aggregation. Biotechnol Appl Biochem 1998 Oct;28 ( Pt 2):119-24. 5. Tissue and cell type specificity of the human neurotrophin-3 promoter region in transgenic mice. Brain Res Mol Brain Res 1998 Aug 15;59(1):114-8. 6. Human neurotrophin-3: a one-step peptide mapping method and complete disulfide characterization of the recombinant protein. \nJ Protein Chem 1996 May;15(4):351-8.