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Nuclear Cytosol Fractionation BioAssay(TM) Kit

Cat no: 167926

Nuclear Cytosol Fractionation BioAssay(TM) Kit

Nuclear/Cytosol Extraction BioAssay(TM) Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. \n\nSample Type:\nMitochondria, mitochondrial extract or cell lysate.\n\nIntended Use:\nThis kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. Transcriptional activity/reporter assays/enzyme activity assays/Western blotting and more downstream assays.\n\nKit Components:\nCytosol Extraction Buffer A (CEB-A), 1x5ml\nCytosol Extraction Buffer B (CEB-B), 1x300ul\nNuclear Extraction Buffer A (NEB), 1x2.5ml\nDTT, 1M, 1x100ul\nProtease Inhibitor Cocktail, 1x vial\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167926

Size

25Tests

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