OAT 4, Control Peptide, Human (Organic anion transporter 4)

Control Peptide for O3003-31 (antiserum) and O3003-32 (affinity purified)\n\nSynthetic peptide, 18-aa, Human OAT4 (1).\n\nMammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).\n\nMost recently, a novel member of the OAT family, OAT4, has been cloned and characterized. OAT4 (human 550 aa) is ~38-44% identical to other OATs. It is prominently expressed in the kidney and placenta. OAT4 interacted with chemically heterogeneous anionic compounds (nonsteroidal antiinflammatory drugs, diuretics, sulfobromopthalein, penicillin G, and bile acid salts). OAT4 may help in eliminating harmful anionic substances and protect the fetus.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1ug/ml\nAntibody Blocking: 5-10ug/1ul O30003-31 or 1ug O3003-32 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O3003-30

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

Purity

Highly purified

References

1. Zhou F, 2004, Mol. Pharmacol., 65:1141-1147, WB 2. Ugele B, 2003, Am J Physiol. Endocrinology Metab: 284, 390-398. IHC 3. Cha SH et al (2000) J. Biol. Chem. 275, 4507-4512.

Additional Info

Sequences from other species are not yet available. No significant sequence homology is detected with other OATs or other proteins. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments to confirm antibody specificity