OATP3, Rat, Control Peptide (Organic Anion Transporting Polypeptide 3)

Control peptide for O3002-06 (antiserum) and O3002-07 (affinity purified antibody).\n\nMammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N- and C-terminus). Oatp3 (670 aa; 12 TM), cloned from rat retina, shares ~80-83% identity with oatp1-2 and ~77% to rat OAT-K1. Oatp3 is highly expressed in the kidney, and moderately in the retina. Oatp3 mediates uptake of taurocholate, thyroxine, and triiodothyronine. Most of the T4 secreted from the thyroid is deiodinated in peripheral tissues. The liver and kidney are the major sites for the production of T3 and T4. Therefore, oatp3 may play a critical role in transporting thyroid hormones from the circulation to the deiodination sites in the kidney.\n\nApplications: \nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: Coat at 1ug/ml.\nAntibody Blocking: 5-10ug per 1ul O3002-06 (antiserum) or per 1ug O3002-07 (affinity purified antibody).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O3002-05

Size

50ug

Applications

ELISA

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide

Purity

Highly purified

References

1. Abe, T., et al., J. Biol. Chem. 273: 22395-22401 (1998). 2. Noe, B., et al., PNAS 94: 10346-10350 (1997). 3. Abe, T., et al., J. Biol. Chem. 273: 22395-22401 (1998). 4. Kakyo, M., et al., FEBS Lett. 445: 343-346 (1999).

Additional Info

Synthetic peptide consisting of 12aa sequence within the mid-region of rat Oatp3. No significant sequence homology with other Oatp2 and Oatp3. Species Sequence Homology: Mouse Oatp3: 75%. Human Oatp or LST1: No significant sequence homology.