Oct-1 (Organic Cation Transporter 1)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient- dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues. Oct superfamily of proteins share a high degree of sequence homology and display up to 12 transmembrane domains with cytoplasmic N- and C-terminus. Oct1 (rat/mouse 556aa, ~95% homology; human 554aa, ~78% homology with rat Oct1) is expressed primarily in the kidney, liver and intestine. Rat Oct1 has been localized to the basolateral membrane of small intestinal enterocytes, hepatocytes, and S1 segment of the proximal renal tubules. Oct1 mediated uptake of TEA was pH and Na+-independent and was reduced when membrane potential decreased. Oct1 also transported NMN, choline, MPP, dopamine, thiamine, noradrenaline, histamine, and spermine but not for putrescine.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.\nWestern Blot: 1-10ug/ml (ECL). Band at ~66kD (rat kidney).\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nO5400-05: Oct-1, Rat, Control Peptide\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

O5400-06

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Grundemann, D., et al., Nature (Lond.) 372: 549-552 (1994). 2. Zhang, L., et al., JBC 272: 16548 (1997). 3. Zhang, L., et al., Mol. Pharmacol. 51: 913 (1997). 4. Gorbuleve, V., et al., DNA Cell. Biol. 16: 871 (1997). 5. Koespell, H., Ann. Rev. Physiol. 60: 243-266 (1998, review). 6. Urakami, Y., et al., J. Pharmacol. Expt. Therap. 287: 800-805 (1998). 7. Meyer-Wentrup, F., et al., BBRC 248: 673-678 (1998).

Additional Info

Recognizes rat Oct-1 (Organic Cation Transporter 1). The 21aa rat control peptide is 100% conserved in rat Oct1a (430aa), an alternatively spliced form of rat Oct1 (1). No significant sequence homology is detected with other Octs or OctNs.