Okadaic acid sodium salt (high purity) (Halochondrine A, CCRIS 3329, HSDB 7243, 9,10-Deepithio-9,10-didehydroacanthifolicin)

Non-phorbol type tumor promoter. Reversible, potent and selective serine threonine protein phosphatase inhibitor. PP2A (IC50=0. 2-1nM), PP1 (IC50=3-15nM), PP2B (IC50=>1microM). Does not inhibit PP2C. Stimulates intracellular protein phosphorylation. Useful tool for studying cellular processes that are regulated by phosphorylation. Does not affect activity of acid phosphatase, alkaline phosphatase and tyrosine phosphatase. Mimics the effects of insulin. Activates atypical protein kinase C (zeta/lambda) in 3T3/L1 adipocytes. Enhances transmitter release at neuromuscular junctions. Apoptosis inhibitor. Induces apoptosis in human breast carcinoma cells (MB-231 and MCF-7) and in myeloid cells. Neurotoxic. Used to study various cellular processes including cell cycle, apoptosis, nitric oxide metabolism and calcium signaling. Stimulates cell motility, loss of stabilization of focal adhesions and a consequent loss of cytoskeletal organization. \n\nStorage and Stability:\nShort-term Storage: +4 degrees C\nLong-term Storage: -20 degrees C\nStable for at least 2 years after receipt when stored at -20 degrees C.

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SPECIFICATIONS

Catalog Number

045117

Size

25ug

Purity

>98% (HPLC)

References

Product Reference: \nOkadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter: M. Suganuma, et al.; PNAS 85, 1768 (1988) ?\nInhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics: C. Bialojan & A. Takai; Biochem. J. 256, 283 (1988) ?\nEffects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism: T.A. Haystead, et al.; Nature 337, 78 (1989) ?\nAn improved procedure for identifying and quantitating protein phosphatases in mammalian tissues: P. Cohen, et al.; FEBS Lett. 250, 596 (1989) ?\nOkadaic acid: a new probe for the study of cellular regulation: P. Cohen, et al.; TIPS 15, 98 (1990) (Review) ?\nUse of okadaic acid to inhibit protein phosphatases in intact cells: D.G. Hardie, et al.; Methods Enzymol. 201, 469 (1991) ?\nEffects of okadaic acid, an inhibitor of protein phosphatases-1 and -2A, on glucose transport and metabolism in skeletal muscle: J.F. Tanti, et al.; J. Biol. Chem. 266, 2099 (1991)?\nProtein phosphatase inhibitor okadaic acid enhances transmitter release at neuromuscular junctions: M. Abdul-Ghani, et al.; PNAS 88, 1803 (1991)?\nInhibition of apoptosis in human tumour cells by okadaic acid: Q. Song, et al.; J. Cell Physiol. 153, 550 (1992) ?\nDifferential induction of apoptosis in human breast tumor cells by okadaic acid and related inhibitors of protein phosphatases 1 and 2A: K. Kiguchi, et al.; Cell Growth Differentiation 5, 995 (1994)?\nDifferential inhibition and posttranslational modification of protein phosphatase 1 and 2A in MCF7 cells treated with calyculin-A, okadaic acid, and tautomycin: B. Favre, et al.; J. Biol. Chem. 272, 13856 (1997)?\nOkadaic acid-induced apoptosis in neuronal cells: evidence for an abortive mitotic attempt: R. Nuydens, et al.; J. Neurochem. 70, 1124 (1998)?\nInhibitors of protein phosphatase 1 and 2A differentially regulate the expression of inducible nitric-oxide synthase in rat astrocytes and macrophages: K. Pahan, et al.; J. Biol. Chem. 273, 12219 (1998)?\nOkadaic acid activates atypical protein kinase C (zeta/lambda) in rat and 3T3/L1 adipocytes. An apparent requirement for activation of Glut4 translocation and glucose transport: M.L. Standaert, et al.; J. Biol. Chem. 274, 14074 (1999)?\nUnique features of the okadaic acid activity class of tumor promoters: H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol. 125, 150 (1999) (Review)?\nNeurotoxic and synaptic effects of okadaic acid, an inhibitor of protein phosphatases: R. Tapia, et al.; Neurochem. Res. 24, 1423 (1999) (Review)?\nOkadaic acid, useful tool for studying cellular processes: J.J. Fernández, et at.; Curr. Med. Chem. 9, 229 (2002) (Review)?\nOkadaic acid: the archetypal serine/threonine protein phosphatase inhibitor: A.B. Dounay & C.J. Forsyth; Curr. Med. Chem. 9, 1939 (2002) (Review) ?\nEffect of okadaic acid on glucose regulation: M.C. Louzao, et at.; Mini Rev. Med. Chem. 5, 207 (2005) (Review)?\nMarine toxins and the cytoskeleton: okadaic acid and dinophysistoxins: C. Vale & L.M. Botana; FEBS J. 275, 6060 (2008) (Review)?\n

Alternative Names

Halochondrine A, CCRIS 3329, HSDB 7243, 9,10-Deepithio-9,10-didehydroacanthifolicin