Omi, HtrA2, Control Peptide (HtrA serine peptidase 2)

HtrA2/Omi is a mammalian serine protease at high temperatures and a chaperone at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.\n\nHtra2 is a novel member of the Htra serine protease family. Human Htra2 is localized in the mitochondria and is involved in mammalian stress response pathways. Human Htra2, predominantly a nuclear protease, is upregulated in mammalian cells under stress, due to heat shock or tunicamycin treatment. Htra2 plays a role in regulating apoptosis by binding and inhibiting caspases.\n\nThe inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity (1-3). Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP. Like Smac/DAIBLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N-terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway. \n\nLocation: \n15aa near the C-terminus of human OMI (1).\n\nApplications: \nThe peptide is used for blocking the activity of OMI (Htr2A) antibody. It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37 degrees C. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O6350-06A

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Purity

Purified

References

1. Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174. 2. Liston P, Fong WG, and Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80. 3. Vaux DL and Silke J. Mammalian mitochondrial IAP binding proteins. Biochem. Biophys. Res. Comm. 2003; 304:499-504. 4. Suzuki Y, Imai Y, Nakayama H, et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 2001; 8:613-21. 5. Yang QH, Church-Hajduk R, Ren J, et al. Omi/HtrA2 catalytic cleavage of inhibitor of apoptosis (IAP) irreversibly inactivates IAPs and facilitates caspase activity in apoptosis. Genes Dev. 2003; 17:1487-96. 6. Miller, L., et al., Trends Cell Biol. 9: 323-328 (1999). 7. Zumbrunn, J., et al., FABS Lett. 398: 187-192 (1996). 8. Gui-Ying, N., et al., Biochem. J. 371: 39-48 (2003). 9. Miller LK. Trends Cell Biol. 9: 323-328 (1999). 10. Deveraux QL, and Reed JC. Gense Dev. 13: 239-252 (1999). 11. Reed JC and Bischoff JR Cell 102: 545-548 (2000). 12. Hegde R, Srinivasual SM, Zhang J, Wasell R, et al. J. Biol. Chem 277: 432-438 (2002). 13. Sug Hyung Lee, et al. Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer. Apmis Volume 111 ( 5): 586 (2003).