Omi, HtrA2, Recombinant, Human (HtrA serine peptidase 2)

HtrA2 also called Omi is a mammalian serine protease at high temperatures and has a chaperone activity at low temperature. The full-length HtrA2 is synthesized as a precursor protein and then targeted to the mitochondria where it is matured by the removal of N-terminal 133 residues. Mature HtrA2 consists of a putative transmembrane domain; an inhibitor of apoptosis protein (IAP)-binding motif; a single C-terminal PDZ domain that mediates protein-protein interactions. Recently, HtrA2 has known to contribute both to caspase-dependent and caspase-independent cell death.\n\nRecombinant Human HtrA2 amino acids 134-458 His-Tag fusion proein produced in E.Coli is a single,non-glycosylated polypeptide chain having a molecular mass of 32kD.\n\nSequence: \nMAVPSPPPAS PPSQYNFIAD VVEKTAPAVV YIEILDRHPF LGREVPISNG SGFVVAADGL IVTNAHVVAD RRRVRVRLLS GDTYEAVVTA VDPVADIATL RIQTKEPLPT LPLGRSADVR QGEFVVAMGS PFALQNTITS GIVSSAQRPA RDLGLPQTNV EYIQTDAAID FGNAGGPLVN LDGEVIGVNT MKVTAGISFA IPSDRLREFL HRGEKKNSSS GISGSQRRYI GVMMLTLSPS ILAELQLREP SFPDVQHGVL IHKVILGSPA HRAGLRPGDV ILAIGEQMVQ NAEDVYEAVR TQSQLAVQIR RGRETLTLYV TPEVTEGSHH HHHH\n\nStorage and Stability:\nFor long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O6350-10

Size

10ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 50mM sodium chloride, 1mM DTT, 20% glycerol.

Purity

(same/more than) 90% Determined by Analysis by RP-HPLC. or reducing and non-reducing SDS-PAGE Coomassie.

References

1. [Effects of Omi/HtrA2 on Expression of Anti-apoptotic Protein PED/PEA-15 and Apoptosis of Prostate Cancer Cell Line PC-3.] Ai Zheng 2006 Jun;25(6):677-82 2. Immunohistochemical analysis of Omi/HtrA2 expression in prostate cancer and benign prostatic hyperplasia. J Huazhong Univ S ci Technolog Med Sci 2005;25(6):671-3 3. The protease inhibitor Ucf-101 induces cellular responses independently of its known target, HtrA2/Omi. Cell Death Differ 2006 May 12; 4. The inhibitor-of-apoptosis protein Bir1p protects against apoptosis in S. cerevisiae and is a substrate for the yeast homologue of Omi/HtrA2. J Cell Sci 2006 May 1;119(Pt 9):1843-51 5. HtrA2 is up-regulated in the rat testis after experimental cryptorchidism. Int J Urol 2006 Feb;13(2):157-64 6. The homotrimeric structure of HtrA2 is indispensable for executing its serine protease activity. Exp Mol Med 2006 Feb 28;38(1):36-43