Orexin 2 Receptor STEP Reporter Assay Kit (Luminescence)

Cayman's STEP Reporter Assays (Luminescence) consist of a 96-well plate pre-coated with a transfection complex consisting of the gene for the protein target of interest (i.e., an assay specific target protein), a SEAP reporter construct, and, in some cases, other genes needed to make the assay function with high fidelity. The plates are provide sealed and ready to use - all you need to do is add your cells. Transfection occurs overnight; cells can be tested the next day. The SEAP reporter is secreted into the media, so cellular responses can be followed over time. The kits are easily adapted to high throughput screening.

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SPECIFICATIONS

Catalog Number

600250

Form

1 ea

P Type

Assay Kits|STEP Assays

Weight

0

Storage Temp

-80

Shipping Temp

-80

Additional Info

The Orexin 2 Receptor (OX2R) may be an important therapeutic target for treatment of sleep disorders, obesity, emotional stress, and addiction. Cayman's Orexin 2 Receptor STEP Reporter Assay Kit (Luminescence) consists of a 96-well plate coated with both OX2R and Secretory Alkaline Phosphatase (SEAP) reporter constructs (OX2R STEP Plate). Cells grown on the STEP complex will express OX2R at the cell surface. Binding of agonists to OX2R initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture medium. Aliquots of culture medium are removed at time intervals beginning at about 24 hours and SEAP activity is measured following addition of a luminescence-based alkaline phosphatases substrate provided in the kit. The kit is easy to use and can be easily adapted to high throughput screening for therapeutic compounds regulating activation of OX2R. A known OX2R agonist, Orexin A, is included in the kit for use as a positive control. The kit provides sufficient reagent to measure SEAP activity at three time points, using the black plates provided.