Oxgall is a purified, dried form of ox bile used in bacteriological media formulations for gastro-intestinal tract organisms, eg, enterocossel agar. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials. Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens. Oxbile is also found in Littman Agar, a selective fungal medium.
Oxbile is used as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Gram-positive bacteria. The major composition of Oxbile is taurocholic and glycocholic acids.
Appearance: Greenish to light beige or brown, homogeneous powder
Solubility (2%): Gold/greenish to amber, clear, complete
pH (2%): 7.0-8.5
Loss on Drying: (same/less than)6%
Total Bile Acids: (same/more than)50%
Storage and Stability:
Store powdered media at 4 degrees C.Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degrees C and used within a short period of time. Protect from moisture and light by keeping container tightly closed.
Quick Search
Products 
