Cayman's Oxygen Consumption/Mitochondrial Membrane Potential Dual Assay Kit is a multiplex assay designed to provide a comprehensive picture of mitochondrial performance in living cells. This assay simultaneously measures both oxygen consumption rate and mitochondrial membrane potential by utilizing MitoXpress(R) - Xtra, a phosphorescent oxygen probe, and the cationic dye, JC-1. Antimycin A, an inhibitor of the mitochondrial electron transport chain, is included to be used as a positive control. Additionally, glucose oxidase is provided as a reference for oxygen depletion. This easy-to-use kit can be adapted for high throughput screening of compounds that modulate mitochondrial function.