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p13 suc1 (Agarose)

Cat no: P0999

p13 suc1 (Agarose)

Recombinant yeast GST-p13 suc1 GST fusion protein, Mr~39kD, non-covalently bound to glutathione-agarose.\n\nSDS-PAGE and Coomassie Stain: 2ug of p13 suc1 Agarose was separated by SDS-PAGE and stained with coomassie blue.\n\nApplications:\nSuitable for use in cdk1/cdc2 precipitation. Other applications not tested.\n\nRecommended Dilution: \nImmunoprecipitation: 25-100ug precipitated cdk1/cdc2 kinase from 500ug of a HeLa cell lysate, as confirmed by Western Blot analysis of the precipitate with C2571. A previous lot precipitated cdk1/cdc2 kinase from a lysate of Sf9 cells that over-expressed cdk1/cdc2, as confirmed by Western Blot analysis of the precipitate with C2571-06.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P0999

Size

2.5mg

Applications

IP

Conjugates

Agarose

Form

Supplied as a liquid suspension in PBS, 0.05% sodium azide, 50% glycerol.

Purity

(same/more than) 95% by SDS-PAGE and Coomassie Blue Staining

References

1. Draetta, D., et al., Cell 50: 319

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