p130Cas (Cas for Crk-associated substrate, BCAR1, K-associated Substrate, Breast Cancer anti-estrogen Resistance 1 Protein)

p130Cas (protein 130kD Crk-associated substrate; also BCAR1) is a cytoplasmic member of the CAS family of proteins. Although its predicted MW is 93kD, it runs anomalously at 105-130kD in SDS-PAGE. It is found in a wide variety of cell types. p130Cas is involved in cell spreading and migration (but not adhesion), and its cleavage into a 31kD fragment promotes anoikis and cell death. Human p130Cas is 870aa in length. It contains one SH3 domain (aa3-65), a Pro-rich region (aa74-87), at least 10 phosphotyrosine (aa128-410) and four phosphoserine (aa134-639) sites, an SH3 binding motif (aa635-643) and a HLH dimerization segment that interacts with CASL.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P1001-97B

Size

100ug

Applications

WB

Hosts

Sheep

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes endogenous human p130Cas at 105-130kD. Species Crossreactivity: mouse, rat.