P38delta, Active, Recombinant, Human (Active P38delta, P38delta, P38, p38, p38delta, p38d, human P38delta, active kinase, active kinases, recombinant P38delta, recombinant P38d, kinase activity assay, protein kinase)

Mitogen-activated protein kinase (MAPK) cascades represent one of the major signal systems used by eukaryotic cells to transduce extracellular signals into cellular responses, and involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The stress-activated protein kinase 4 (SAPK4), or p38 delta, is a member of the MAPK family that are activated by chemical and environmental stresses as well as by proinflammatory cytokines. SAPK4 has a TGY dual phosphorylation motif and is activated in response to cellular stresses and proinflammatory cytokines. MAP kinase kinases 3, and 6 can phosphorylate and activate this kinase. Transcription factor ATF2, and microtubule dynamics regulator stathmin have been shown to be the substrates of this kinase.\n\nSource:\nRecombinant protein corresponding to human p38delta, expressed in Sf9 insect cells.\n\nMolecular Weight: \n~71.0kD\n\nBiological Activity:\n259 nmol phosphate incorporated into MBP protein per minute per mg protein at 30 degrees C for 15 minutes using a final concentration of 50um ATP.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

167931

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

~80% (SDS-PAGE)

References

1. Goedert, M.; Cuenda, A.; Craxton, M.; Jakes, R.; Cohen, P. Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6); comparison of its substrate specificity with that of other SAP kinases. EMBO J. 16: 3563-3571, 1997. 2. Kumar S, McDonnell PC, Gum RJ, Hand AT, Lee JC, Young PR. Novel homologues of CSBP/p38 MAP kinase: activation, substrate specificity and sensitivity to inhibition by pyridinyl imidazoles. Biochem Biophys Res Commun. 1997 Jun 27;235(3):533-8.