The tumor suppressor protein p53 plays a crucial role in coordinating cellular responses to genotoxic stress and holds many important clinical implications in the treatment of cancer. Cayman's p53 Cell-Based Activation/Translocation Assay provides a highly specific p53 primary monoclonal antibody together with a DyLight(TM) (product of Thermo Scientific) conjugated secondary antibody in a ready-to-use format. (-)-Nutlin-3, a potent inhibitor of Mdm2-p53 interaction, which has been shown by scientists at Cayman to cause the activation and translocation of p53 between the cytoplasm and nuclear compartments, is included as a positive control.