p53, Recombinant, Human (Cellular Tumor Antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor Suppressor p53, TP53)

p53 is expressed in a wide variety of tissues and plays a major role in preventing tumour development. It is involved in cell cycle regulation, functioning as a trans-activator to negatively regulate cell division by controlling a set of genes required for this process. It acts as a tumour suppressor in many tumour types by stimulating either apoptosis or growth arrest depending on the physiological circumstances and the cell type. Defects in p53 expression have been implicated in several diseases including; choroid plexus papilloma, lung cancer, head/neck squamous cell carcinomas, oesophageal squamous cell carcinoma, Li-Fraumeni syndrome, and hereditary adrenocortical carcinoma.\n\nSource:\nRecombinant corresponding to full length human p53, N-terminal c-Myc, 6His-tagged expressed in baculovirus in Sf21 insect cells.\n\nMolecular Weight:\n~49kD\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029572

Size

10ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 0.3M sodium chloride, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Purity

~94% (SDS-PAGE and Coomassie blue staining)

References

1. Matlashewski G. et al., Isolation and Characterization of a Human p53 cDNA Clone: Expression of the Human p53 Gene. EMBO J. 3: 3257-3262, 1984. 2. Hock A. and Vousden K.H. Regulation of the p53 Pathway by Ubiquitin and Related Proteins. Int J Biochem Cell Biol. 42: 1618-1621, 2010. 3. Hollstein M. et al., p53 Mutations in Human Cancers. Science. 253: 49-53, 1991. 4. Petitjean A. et al., Impact of Mutant p53 Functional properties on TP53 Mutation Patterns and Tumor Phenotype: Lessons From Recent Developments in the IARC TP53 Database. Hum Mutat. 28: 622-629, 2007. 5. Kruse J-P. and Gu W. Modes of p53 Regulation. Cell, 137: 609-622, 2009.