PACSIN3 (Protein Kinase C And Casein Kinase Substrate In Neurons Protein 3, SH3 Domain-containing Protein 6511, Endophilin I)

A member of the Endophilin family, Endophilin A1 is a protein containing SH3 domains, and has been implicated in the trafficking of clathrin-mediated synaptic vesicle endocytosis within the membrane. It was originally characterized as a presynaptically enriched protein that binds to endocytic proteins polyphosphoinositide synaptojanin and dynamin. Endophilin A1 has been implicated in endocytic vesicle budding when its function in cell-free systems and in living synapses has been disturbed. Along with the SH3 domain, Endophilin A1 contains a BAR domain in the N-terminal region, that has been found to induce and bind membrane curvature. This protein's expression is localized to the brain, most specifically in the frontal cortex. Markedly high expression is observed in fetal cerebellum.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P1793-10F

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Cuajungco MP, Grimm C, Oshima K, D\\1hoedt D, Nilius B, Mensenkamp AR, Bindels RJ, Plomann M, Heller S. PACSINs bind to the TRPV4 cation channel. PACSIN 3 modulates the subcellular localization of TRPV4. J Biol Chem. 2006 Jul 7;281(27):18753-62. Epub 2006 Apr 20.

Additional Info

Recognizes human PACSIN3. Species sequence homology: Mouse and rat.