Paired Immunoglobulin-Like Type 2 Receptor beta, Recombinant, Mouse (PILRb)

PILR-Beta (paired immunoglobulin-like type 2 receptor-beta) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-alpha and it likely arose through PILR-alpha gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-Beta and -alpha should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-Beta and -alpha show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-Beta is a 196 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a short, eight aa cytoplasmic region. The ECD shows a V-type Ig-like domain (aa 39-135), while the TM segment contains a positively-charged Lys at position # 202. This Lys is known to interact with the transmembrane signaling adaptor protein DAP12, making PILR-Beta an activating receptor. Activation of PILR-Beta through CD99 ligation induces NK cell cytotoxicity and dendritic cell secretion of NO and TNF-alpha (3). Mouse PILR-Beta is found on NK cells, neutrophils, macrophages, and monocyte-derived dendritic cells (3). Mouse PILR-Beta ECD is 44% and 75% aa identical to human and rat PILR-Beta ECD, respectively; it is 75% aa identical to the ECD of mouse PILR-alpha (3). Evidence suggests that mouse PILR-Beta will not be active in a human system (3). Potential isoforms of PILR-Beta have been reported. One shows an alternate start site at Met14, a second shows a 21 aa substitution for the C-terminal 67 aa, and a third exhibits multiple polymorphisms for an overall aa identity of 86% (4, 5, 6).\n\nSource: A DNA sequence encoding the extracellular domain of mouse PILR-Beta (Ala 15-Gly 180; Accession # NP_573472) was fused to a 6x histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.\n\nDefinition: Measured by its ability to bind rmCD99/mFc.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 19.6kD migrates as an ~ 39-44kD protein in SDS-PAGE under reducing conditions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P2150-50E

Size

50ug

Applications

WB

Reactivities

Mouse

Form

Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to (same/more than) 0.1mg/ml.

Purity

(same/more than) 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin (same/less than) 1EU.

References

1. Wilson, M.D. et al. (2006) Physiol. Genomics 27:201.\n2. Lanier, L.L. (2001) Curr. Opin. Immunol. 13:326.\n3. Shiratori, I. et al. (2004) J. Exp. Med. 199:525.\n4. Genbank Accession # NP_573472.\n5. Genbank Accession # BAC29442.\n6. Genbank Accession # XP_001480583.